(1)Mixing corn meal and water into paste, putting the paste into boiled water, then boiling and stiring for 1 hr., making it in5% concentration, distributing into tubes, autoclave at 1.5kg/cm square for 90 min. for pastification

(2)Inoculation with bacteria spore in sand when cold

(3)After inoculation, putting the tubes in boiling water bath for 1-2 min, for heat treatment

(4)Cooling the tubes in top water immediately

(5)Putting the tubes in vacuum desicator and pumping it to vacuum

(6)Incubation at 37C for 3 days, when the surface of the broth floated with soild material and the cloud-like suspended in

the lower part of the tube, the cultures are growth well