Int J Syst Evol Microbiol. 2017 Apr;67(4):993-997. doi: 10.1099/ijsem.0.001730. Epub 2017 May 5.

Fang XM1, Su J1, Wang H1, Zhang T1, Zhao LL1, Liu HY1, Ma BP2, Zhang YQ1, Yu LY1.

Author information

1 Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, PR China.

2 ​Beijing Institute of Radiation Medicine, Beijing, PR China.

Abstract

The taxonomic status of a novel bacterium, designated strain CPCC 100226T, isolated from a traditional Chinese medicinal herbal plant, Eucommia ulmoides Oliver, was characterized by using a polyphasic approach. The aerobic isolate formed pale white colonies on tryptic soy agar. Cells were Gram-stain-positive, rod-shaped, motile and endospore-forming. Chemotaxonomic investigations revealed the presence of meso-diaminopimelic acid as the diagnostic diamino acid, MK-7 as the predominant menaquinone, anteiso-C15 : 0, iso-C15 : 0, iso-C16 : 0 and C16 : 0 as the major fatty acids, and the strain had a phospholipid pattern of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and unidentified aminophospholipids. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the isolate was closely related to Paenibacillus aestuarii DSM 23861T with 95.1 % similarity. The G+C content of the genomic DNA was 47.9 mol%. On the basis of the genotypic and phenotypic data, the isolate is considered to represent a novel species of the genus Paenibacillus. The name proposed for this taxon is Paenibacillus eucommiae sp. nov. with CPCC 100226T (=DSM 26048T=KCTC 33054T) as the type strain.

PMID:27959785  DOI:10.1099/ijsem.0.001730

https://www.ncbi.nlm.nih.gov/pubmed/?term=Paenibacillus+eucommiae+sp.+nov.%2C+isolated+from+a+traditional+Chinese+medicinal+herbal+plant%2C+Eucommia+ulmoides+Oliver