Casein peptone, tryptic digest  10.00 g

Meat extract                    10.00 g

Yeast extract                   5.00 g

Glucose                         20.00 g

Tween 80                        1.00 g

K2HPO                           42.00  g

Na-acetate                      5.00 g

(NH4)2citrate                   2.00 g

MgSO4x 7 H₂O                    0.20  g

MnSO4x H₂O                      0.05  g

Distilled water                1000.00 ml

Adjust pH to 6.2 - 6.5.